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1 edition of Single molecule spectroscopy and imaging found in the catalog.

Single molecule spectroscopy and imaging

J. Enderlein


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Single molecule spectroscopy and imaging by J. Enderlein Download PDF EPUB FB2

Thomas Basché is the editor of Single-Molecule Optical Detection, Imaging and Spectroscopy, published by Wiley. William Esco Moerner is an American physical chemist and chemical physicist with current work in the biophysics and imaging of single molecules.

He is credited with achieving the first optical detection and spectroscopy of a single molecule in condensed. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging.

Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which startedin physics, chemistry and biosciences is displayed.

The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and.

The workshop on "Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences" is an annual event since For a summary of each year's event, please select the year from the list below.

As ofit has been 26 years since the first optical detection and spectroscopy of single molecules in condensed matter.

This area of science has expanded far beyond the early low temperature studies in crystals to include single molecules in cells, polymers, and in solution. The early steps relied upon Single-Molecule Microscopy and SpectroscopyCited by: The second section covers the use of near-field scanning optical microscopy (NSOM) for single-molecule studies at room temperature.

Trautman and Ambrose first discuss the fundamental principles of NSOM and then describe its applications 5/5(1). Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules - Kindle edition by Sauer, Markus, Hofkens, Johan, Enderlein, Jörg.

Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Handbook of Fluorescence Spectroscopy and Imaging: From Cited by: This book surveys recent advances related to the application of single molecule techniques in various fields of science.

The topics, each described by leading experts in the field, range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Introduction. Single molecule spectroscopy (SMS) has undergone a remarkable development from its origins as a technique in low-temperature high-resolution spectroscopy [, ] to applications in super-resolution fluorescent microscopy [].Its ability to unmask the unique features of individual molecules has found use in low-temperature physics and optics [, ], in research of soft matter Cited by: 4.

Highly sensitive absorption spectroscopy is used to image the presence of single molecules through their weak optical absorption signatures. Measurements are Cited by: Lecture Single-Molecule Imaging and Spectroscopy by NSOM How does single-molecule measurement work.

Why at single-molecule level. Broad applications of single-molecule measurement. NSOM imaging at single- molecule level high spatial resolution (up to 10 nm). Robert Dunn, Chem. Rev. vol, File Size: 2MB. Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap.

Starting with the basic principles, the book goes on to treat fluorophores and labeling. Verification of single-molecule (SM) detection for surface-enhanced Raman spectroscopy (SERS) requires the use of two analytes via either the bianalyte or isotopologue approach. For both approaches, the preferential observation of the individual analytes over a combination of both analytes is used to conclude that SM detection has been by: Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependent Transcription Factor Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy.

Pages Book Title Single Molecule Microscopy in Neurobiology Editors. Nobuhiko. Single-molecule (SM) surface-enhanced Raman spectroscopy (SERS) and tip-enhanced Raman spectroscopy (TERS) have emerged as analytical techniques for characterizing molecular systems in nanoscale environments.

SERS and TERS use plasmonically enhanced Raman scattering to characterize the chemical information on single molecules. Additionally, TERS Cited by: Finally, when combined with scanning probe microscopy (SPM), single molecule spectroscopy allows the measurement of mechanical or electrical properties of single molecules: binding forces, forces holding together secondary and tertiary structure of molecules, torque, bond strength, conductivity, etc.

This article reviews single-molecule spectroscopy and microscopy (SMS) techniques and applications, focusing mainly on the fluorescence emanating from a single emitter (fluorophore). After a historical overview presented in Section 2, the principles underlying single-molecule fluorescence spectroscopy approaches are introduced in Section 3.

Single molecule imaging combined with TIRF has been used to create superresolution images with a resolution of 10 to 20 nanometers. Recommended Literature Peterman, E. G., Sosa, H. and Moerner, W. Single-molecule fluorescence spectroscopy and microscopy of.

The initial steps toward optical detection and spectroscopy of single molecules arose out of the study of spectral hole-burning in inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral signatures relating to the fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in.

A versatile experimental approach is single-molecule spectroscopy in combination with Förster resonance energy transfer and nanosecond fluorescence correlation spectroscopy.The development of fluorescence spectroscopy and imaging on the single molecule level has provided a new method to investigate various phenomena in Author: William Esco Moerner.The NOOK Book (eBook) of the Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules by Markus Sauer, Johan Hofkens, Jörg Due to COVID, orders may be delayed.

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